Extended Data Fig. 8: Localization of CD8+ CNS-associated T cell subtypes. | Nature Aging

Extended Data Fig. 8: Localization of CD8+ CNS-associated T cell subtypes.

From: Accumulation of cytotoxic T cells in the aged CNS leads to axon degeneration and contributes to cognitive and motor decline

Extended Data Fig. 8

a, Immunofluorescent localization of CD8+ T cells (arrows) and laminin+ blood vessels in longitudinal optic nerve and coronal brain sections of adult (6-month-old) CXCR3-GFP reporter mice. CD8+ T cells associated with the CNS parenchyma, meninges, choroid plexus and cerebrospinal fluid express CXCR3, while intravascular and circulating T cells are negative for CXCR3. Scale bar, 10 μm. b, Immunohistochemical characterization of CD8+ T cells (arrows) in distinct CNS-compartments of aged (24-month-old) Wt and adult CX3CR1-GFP or CXCR3-GFP reporter mice. Parenchymal (Paren) and meningeal (Men) CD8+ T cells (clusters 1 to 5) show heterogeneous expression of Lag3, CD103 and PD-1. A20 and high Stat1 expression is detected in meningeal and choroid plexus (CP)-resident CD8+ T cells (IST). CD8+CD62L-CX3CR1+ TEFF and CD8+CD62L+CXCR3- TCM2 are detected in blood and CD8+CD62L+CXCR3+ TCM1 are detected in meninges and cerebrospinal fluid (CSF; inset). Scale bar, 10 μm. Micrographs represent three independent experiments with similar results.

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