Extended Data Fig. 4: The transfer of OC-exosomes to OA cartilage in vivo. | Nature Aging

Extended Data Fig. 4: The transfer of OC-exosomes to OA cartilage in vivo.

From: Exosomal transfer of osteoclast-derived miRNAs to chondrocytes contributes to osteoarthritis progression

Extended Data Fig. 4: The transfer of OC-exosomes to OA cartilage in vivo.

The representative confocal images of the PKH26-labelled OC-exosomes in tibial cartilage, subchondral bone and metaphysis area in ACLT mice or Sham mice at 2w after surgery. The mice were intravenously injected with OC-exosomes (20 μg per mouse) 3 days before euthanasia. Left: PKH26-labelled OC-exosomes indicated by red fluorescent signal. Middle left: Endomucin+ vessels indicated by green fluorescent signal. Middle right: Merged images with DAPI-stained nucleates (blue) and bright field. Right: 20x zoom-in of the cartilage layer. Dotted lines indicate the osteochondral junction. Arrows indicate the PKH26-labelled OC-exosomes at osteochondral junction and cartilage layer. Scale bar: 100 μm. All experiments were performed in biologically independent triplicates in each group.

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