Extended Data Fig. 5: OC-derived exosomal miR-214-3p promote cartilage matrix degeneration, angiogenesis and sensory axon innervation in vivo.
(a) Schematic diagram showing the strategy for osteoclast-targeted delivery of control ncRNA in Ctsk-Cre mice (WT, OCExo-Intact), osteoclast-specific miR-214 knockout mice (CKO, OCExo-Intact) and osteoclast-specific miR-214 knockin mice (CKI, OCExo-Intact) and osteoclast-targeted delivery of Rab27a siRNA in the CKI mice (OCExo-BLK). (b-c) QPCR analysis of the miR-214-3p in BM-OCs and serum exosomes (b) and the pri-miR-214 and miR-214-3p in cartilage (c) from the indicated mice. (d) The burrowing performance. (e) Left: Representative Safranin O staining images of tibial articular cartilage. Right: the OARSI scores. (f-g) Left: Representative fluorescent images of the Endomucin (EDMC, green) and CD31 (red) immunostaining (f) and CGRP (green) immunostaining (g) at tibial articular cartilage and subchondral bone. Right: the number of EDMChiCD31hi cells (f) and CGRP+ fibers (g) around the osteochondral junction. All scale bar: 100 μm. Data are shown as mean ± s.d. In b & c, N=4 mice per group. The exact p values are shown in figures. Two-way ANOVA with Bonferroni’s (h) multiple comparisons test was used for statistical analysis. In d-g, N=6 mice in each group. One-way ANOVA with Turkey’s multiple comparisons test was used for statistical analysis.
