Extended Data Fig. 6: OC-derived exosomal miRNAs regulate TIMP2 and TIMP3 in chondrocytes in vitro.
(a) Representative confocal fluorescent micrographs showing the co-localization of FAM-labelled miR-214-3p mimics and PKH26-labelled OC-exosomes in chondrocytes. Scale bar: 100 μm. (b) QPCR analysis of the mRNA levels of TIMP1, 2 and 3 in IL-1β-stimulated chondrocytes with or without OCs co-culture. (c) Western blot analysis of the mRNA levels of TIMP1, 2 and 3 in IL-1β-stimulated chondrocytes with or without OCs co-culture. The aforementioned OCs were differentiated from BMMs with either Rab27a siRNA or ncRNA transfection. Data are shown as mean ± s.d. All experiments were performed in biologically independent triplicates in each group. The exact p values are shown in figures. Two-way ANOVA with Turkey’s multiple comparisons test was used for statistical analysis.
