Extended Data Fig. 1: Characterization of cell lineage-dependent response to senescence induction.
a, Immunoblot analysis of gene expression profile after senescence induction of HFL1 cells. Cells were exposed to chemotherapeutic treatment by cisplatin (CIS), carboplatin (CARB), satraplatin (SAT), bleomycin (BLEO), mitoxantrone (MIT) or doxorubicin (DOXO). b, Immunoblot of lysates from HFL1 cells that have experienced replicative senescence, with samples collected at indicated passage numbers. c, Immunoblot of lysates from HFL1 cells transduced with a lentiviral construct encoding control vector or oncogenic Ras (HRasG12V). GAPDH, loading control. d, Immunoblot examination of lysates from human prostate cancer cell lines subject to chemotherapeutic treatment. LNCaP, DU145 and PC3 were exposed to MIT applied at the pre-optimized IC50 concentration per line. e, Immunoblot examination of lysates from human lung cancer cell lines subject to chemotherapeutic treatment. H460, H1299 and A549 were subject to treatment by BLEO at the pre-optimized IC50 concentration per line. f, Immunoblot assay after lentiviral transduction of human p16INK4a to PSC27 stromal cells. SE, short exposure. LE, long exposure. g, Similar to (f), immunoblot assay for HFL1 lentivirally transduced with human p16INK4a to analyze expression of relevant targets. SE, short exposure. LE, long exposure. h, Representative SA-β-Gal staining images of PSC27 and HFL1 upon transduction of HRasG12V or p16INK4a. Scale bar, 20 μm. Right, statistics of staining positivity. i, Quantitative profiling of transcript expression of SASP canonical factors and KDM4 family members. Data in all bar plots are shown as mean ± SD and representative of 3 biological replicates. h (right panel) and i, P values were determined by two-sided unpaired t-test, and adjusted for multiple comparisons.
