Extended Data Fig. 3: Additional characterization of Aβ pathology in old APOE3;5xFAD and APOE4;5xFAD male and female mice and Aβ-independent vascular changes.

a Aβ40 levels in the Ctx and Hipp in E3+FAD (n = 14) and E4+FAD (n = 17) mice. b, Aβ40 levels in the Ctx and Hipp in male and female E3+FAD (n = 13–14, 8–9 ♂ and 5 ♀) and E4+FAD (n = 17, 12 ♂ and 5 ♀) mice. Data are presented as truncated violin plots; continuous line, median; dotted line, interquartile range. c-f, Lack of correlation between the blood-brain barrier (BBB) permeability Ktrans values and Aβ40 levels in the Ctx and Hipp (c,d) and regional cerebral blood flow (CBF) values and Aβ40 levels in the Ctx and Hipp (e,f). Mice (both genders) were 18–24-month old (n = 28 individual points from both groups). In a, significance by unpaired two-tailed Student t-tests. In b, significance by one-way ANOVA followed by the Tukey post hoc test. In c-f, significance by two-tailed simple linear regression; r, Pearson correlation; ns, non-significant.