Extended Data Fig. 4: Effects of Debio-025 relative to vehicle on neuron counts, neuritic density and behavior in APOE4;5xFAD and APOE3;5xFAD mice (red circles, data taken from Fig. 8) compared to the littermate controls without 5XFAD transgenes (blue circles).
a–d, Quantification of NeuN+-neurons (a,b) and SMI312+-neuritic density (c,d) in the Ctx and Hipp in E3 (blue empty circles) and E4 (blue filled circles) untreated littermate controls without 5xFAD transgenes compared to age-matched E3+FAD (red empty circles) and E4+FAD (red filled circles) mice treated with vehicle or Debio-025; In a-d, n = 5 mice per group except in a. n = 4 in for E3 littermate controls without 5XFAD transgenes. e,f, Novel object location (NOL; e) and novel object recognition (NOR; f) in E3 (n = 8, blue empty circles) and E4 (n = 8, blue filled circles) untreated littermate controls without 5XFAD transgenes compared to age-matched E3+FAD vehicle-treated (n = 8; red empty circles), E3+FAD Debio-025-treated (n = 9; red empty circles), E4+FAD vehicle-treated (n = 7; red filled circles), and E4+FAD Debio-025-treated (n = 8; red filled circles) mice. Mice (both genders) were 10–12-month-old. In all graphs, data for E3+FAD and E4+FAD animals are the same as in Fig. 8 (red circles empty and filled); new data used for comparison are from their respective untreated littermate controls without 5XFAD transgenes (blue circles empty and filled). All data are presented as violin plots; continuous line, median; dotted line, interquartile range. Significance by one-way ANOVA followed by the Tukey post hoc test.
