Extended Data Fig. 5: Hair follicle miniaturization and loss in dKO mice.

a, Krt5 and Ki67 staining of hair follicles at early anagen (anagen III, P25) in control and dKO mice, arrowheads indicate Ki67+ HF-SCs, Right panel, quantification of Ki67+ HF-SCs per hair follicle (n=30 hair follicles from 3 pairs of mice). Scale bar, 20μm. b, Ki67 staining of hair follicles at late anagen (anagen V-VI) in control, Foxc1 cKO, Nfatc1 cKO, and dKO mice, arrowheads indicate Ki67+ HF-SCs. Scale bar, 20μm. c, Images of hair coat in the same control and dKO mice on P41 (left panel) and P64 (right panel), the right half of back skin was shaved on P41 and imaged again on P64. d, Images of hair coat of control and dKO mice at ~16mo old. e, Two-photon images of hair follicles in control and dKO mice at 12mo old, red arrowheads point to escaped cells outside of hair follicles. Scale bar, 70μm. f, Boxplot of the percentage of miniaturized hair follicles in young, old and dKO mice. (mean ± s.d.= 0 ± 0 (young), 14 ± 17 (old), 77 ± 25 (dKO); 5 young mice; 3 old mice; 5 dKO mice). g, Representative Two-Photon images for the quantification of HFSCs, red asterisks mark HFSCs(g). h, Boxplot of the number of HFSCs per HF in different samples. (mean ± s.d.= 19.33 ± 2.44 (young), 15.33 ± 4.04 (old), 10.95 ± 3.97 (dKO);91HFs, 5 young mice; 122 HFs, 3 old mice; 58 HFs, 5 dKO mice). i, Longitudinal tracking of dKO hair follicles over 26 days. Red numbers indicate the identical hair follicles in each image. Scale bar, 70μm. j, Boxplot of the percentage of HFs undergo regeneration, degeneration and quiescence in dKO samples (mean ± s.d.= 70 ± 24 (regeneration), 25.23 ± 25 (degeneration), 4.12 ± 7.49 (quiescence); 78 HFs from more than 3 mice were tracked for at least 16 days). Data in a, f, h are two-sided t-test.

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