Extended Data Fig. 4: Differentiation patterns from high-risk mammary epithelial progenitors that had a VUS.

(a-b) Immunofluorescent images of cKit-enriched cells stained for KRT19 (green) and KRT14 (red) that were fixed after 2 days of culture from (a) an HR woman harboring a germline BRCA1 mutation with a BRCA2 VUS and (b) an HR woman harboring a PALB2(VUS) and APC(VUS). (c-d) Density contour plots of KRT14 and KRT19 mean fluorescent intensity signals in cKit-enriched cells from an HR woman harboring germline mutations of (b) BRCA1+BRCA2(VUS) that were either fixed after (c) 2 days or (d) 7 days of culture. Experiments in a-b were repeated twice with similar results. Scale bars = 50 μm.