Extended Data Fig. 3: Ablation of ARF1 in astrocytes, oligodendrocytes, myeloid cells and microglia did not show neurodegenerative phenotypes.
a-g, Body weight, balance beam test and neurological score were assayed in control and cell-type specifically ARF1-deleted mice. a-GFAP-CreER (astrocytes); Arf1f/+: n = 5 mice, 3 male and 2 female mice. Arf1f/f: n = 4 mice, 2 male and 2 female mice. b-Pdgfra-CreER (oligodendrocyte precursor); n = 6 mice, 3 male and 3 female mice. c-Plp1-CreER (oligodendrocytes and schwann cell); n = 6 mice, 3 male and 3 female mice. d-Sox10-CreER (oligodendrocyte lineage cell); n = 5 mice, 2 male and 3 female mice. e-LysM-CreER (myeloid cells); n = 4 mice, 2 male and 2 female mice in control group, and 2 male and 1 female mice in KO group. f-Cx3cr1-CreER (microglia); n = 4 mice, 2 male and 2 female mice in each group. g-Tmem119-CreER (microglia). n = 5 mice, 2 male and 3 female mice. One of three independent experiments was shown. Data are represented as mean ± SEM. h, Western blot for ARF1 expression was performed in the Cre-expressing cell types isolated from Arf1+/- and Arf1-/- mice. Data represents one of three independent experiments. Data are represented as mean with each replicate. P value was calculated using two-tailed t-test.
