Extended Data Fig. 3: The effects of TERT induction on neuroinflammation associated with activation of astrocytes and microglia.

a, Immunohistochemical staining for the astrocytic marker GFAP in the CA1 hippocampal subfield of adult control and Tert-activated R26-CAG-LSL-mTert; 3xTg-AD; Camk2a-CreERT2 mice. Scale bar, 100 μm. b, Quantitative comparison of GFAP-positive astrocytes in the hippocampus (n = 4 per group, 8-month-old, p = 0.0177). c, IBA-1 immunostaining in the CA1 hippocampal subfield of adult control and Tert-activated R26-CAG-LSL-mTert; 3xTg-AD; Camk2a-CreERT2 mice. Scale bar, 100 μm. d, Quantification of IBA1-positive activated microglia in the mouse hippocampus (n = 4 per group, p = 0.0015). Data are mean ± s.e.m. *P < 0.05, **P < 0.01 (two-tailed unpaired t-test).