Extended Data Fig. 4: siRNA knockdown / overexpression of Stat3 and Runx2 in chondrocytes.
From: Kindlin-2 preserves integrity of the articular cartilage to protect against osteoarthritis
(a) Quantitative data for Figure 3e. (b, c) siRNA knockdown of Runx2 and Stat3 in ATDC5 cells. siRNA #1380 (for Runx2) and #1962 (for Stat3) were used for subsequent experiments. (d) Quantitative data for Figure 3r. (e) Quantitative data for Figure 3s. (f) ATDC5 cells were transfected with si-NC or si-Stat3, followed by western blotting for expression of Stat3 and Runx2. (g) ATDC5 cells were transfected with empty vector (EV) and Stat3 expression vector (Stat3), followed by western blotting for expression of t-Stat3, p-Stat3 and Runx2. All experiments were repeated at least three times independently with similar results. Quantitative data are shown as mean ± standard deviation. The exact p values are shown in figures. In a, two-sided unpaired t-test was used for statistical analysis. In d and e, one-way ANOVA with Tukey’s multiple comparisons was used for statistical analysis.
