Extended Data Fig. 6: Lifespan extension upon widespread expression of FOXM1-dNdK transgene (related to Fig. 6).
(a,b) Survival curves of Rosa26 and FoxM1-dNdK males (a) and females (b) under no-dox diet. (c,d) Survival curves of Rosa26 and FoxM1-dNdK males (c) and females (d) under cyclic dox-diet. (e) Mean lifespan (weeks) of the littermates used for survival curve analysis. (f) RT-qPCR analysis of endogenous FoxM1 expression in tissues of 88-week-old (±1.7 y) FoxM1-dNdK (2-ΔΔCt). Tbp and Gapdh were used as reference genes and expression levels were normalized to those in Rosa26 mice tissues. (g) Western blot analysis of FOXM1-dNdK protein levels in extracts of skin, kidney, and liver of 88-week-old mice under dox-diet cyclic induction. Vinculin was used as loading control. (h) Immunostaining of FOXM1-dNdK on skin, kidney and liver sections of the mentioned mice. Scale bars: 100 μm (kidney insets, 50 μm). Error bars represent s.d. “n” represents individual mice throughout, except for (f) where n = independent experiments. Statistics were performed by log-rank (Mantel-Cox) (a-d) and unpaired two-sided t-test (e, f) statistical tests.
