Extended Data Fig. 1: Deep TMT profiling of detergent-insoluble proteome in AD and quantitative analysis of western blotting.
a, Proteomic profiling of detergent-insoluble proteome of 10 control and 10 AD cases by TMT-LC/LC-MS/MS. A total of 8,917 proteins were identified, out of which 365 proteins were found to increase in AD detergent-insoluble proteome. b, Principal component analysis of the insoluble proteome in control and AD cases. c, Heatmap of selected top proteins including U1 snRNP components enriched in AD. d, Pathway enrichment analysis of the proteins enriched in the AD insoluble proteome (Fisher’s exact test and BH procedure to generate FDR values). e, Enriched protein-protein interaction module of spliceosome. f, Quantified data of western blotting (4 replicates) in Fig. 1d (Student’s t-test, two-tailed). g, Quantified data of western blotting (triplicates) in Fig. 1e to show that the N40K expression leads to the depletion of endogenous U1-70K in neurons (Student’s t-test, two-tailed). Data are shown as mean ± SEM. Full statistical information is in Source Data Statistics
