Extended Data Fig. 3: Validation and Morphological and Functional Characterization of LamB and LamC RNAi lines.
Corrected total nuclear fluorescence (CTNF) for cardiomyocytes from (A) LamC RNAi and (B) LamB RNAi fly lines and their respective controls, that is, attp2 and attp40, n = 47, 36, 79, and 69 nuclei from attp2 and LamC RNAi flies at 1 and 4 weeks (left to right). n = 53, 29, 65, and 61 nuclei from attp40 and LamB RNAi flies at 1 and 4 weeks (left to right). (C) Plots quantifying nuclear perimeter (left, n = 111, 96, 46, and 89 nuclei/condition) and aspect ratio (right, n = 113, 97, 46, and 92 nuclei/condition) for LamB, LamC RNAi and their genetic controls. (D) Plots quantifying nuclear area, perimeter, aspect ratio, and circularity (left to right) for LamB and LamC RNAi lines and their genetic control background at 4 weeks. For all plots, n = 136, 101, 86, and 95 nuclei/condition left to right. (E-F) Plots of diastolic and systolic diameters, and shortening velocity determined from SOHA imaging for attp2 control and LamC RNAi flies (E), and attp40 control and LamB RNAi flies (F) at 1 and 4 weeks. Each LamC RNAi plots, n = 21, 23, 25, and 27 nuclei/condition, left to right. Each LamB RNAi plots, n = 31, 21, 26, and 25 nuclei/condition, left to right. For panels A-D, *p < 0.05, **p < 10−2, ***p < 10−3, and ****p < 10-4 from a two-sided unpaired t-test at each time point and RNAi line. For E-F, *p < 0.05, **p < 10−2, ***p < 10−3, and ****p < 10-4 by one-way ANOVA with Tukey multiple comparisons test. Error bars in (A-D) refer to mean + /- SD, and bars in (E, F) refer to min to max, with median and 25th and 75th interquartile range.
