Extended Data Fig. 10: Comparison of mean expression of genes in the aging clocks and genes impacted by rejuvenation in the heterochronic parabiosis and exercise datasets.

a, Scatter plots of the log2 mean normalized BootstrapCell gene expression in the exercise single-cell data as a function of the log2 mean normalized BootstrapCell gene expression in the parabiosis (cohorts 1 and 2 combined) single-cell data for the six main cell types. Red dots correspond to genes that contribute to the chronological aging clocks (selected by clock) and gray dots correspond to genes that do not contribute to the chronological aging clocks (not selected by clock). Genes that contribute to the clock are both highly expressed in the parabiosis and exercise datasets. b, As in (a) but colored dots correspond to genes identified as differentially expressed by parabiosis (green) and exercise (blue). Gray dots correspond to genes that are affected neither by parabiosis nor by exercise (neither). Permissive 1.1-fold change and FDR < 0.1 cutoffs were applied in each of the 2 different conditions.