Extended Data Fig. 7: opt-SNE and FlowSOM for spike-specific CD4+ T cells. | Nature Aging

Extended Data Fig. 7: opt-SNE and FlowSOM for spike-specific CD4+ T cells.

From: Impaired CD4+ T cell response in older adults is associated with reduced immunogenicity and reactogenicity of mRNA COVID-19 vaccination

Extended Data Fig. 7

(a) FlowSOM clustering of AIM+ CD4+ T cells pooled from adults and older adults is displayed on opt-SNE plots. The markers described in the bottom row (CXCR5, CXCR3, CCR6, PD-1, CCR7, CD45RA, CD95, CD57, and CD28) were used for the analysis. AIM+ CD4+ cells were separated into 20 clusters. Th1(TH1), CXCR3+ cTfh, CXCR3āˆ’ cTfh, and N (naĆÆve) cells were manually annotated based on the lineage marker expression. (b) Heatmap showing the marker expression for each FlowSOM cluster.

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