Extended Data Fig. 2: Effect of PI3K inhibitor (PI3Ki) on glucose homeostasis and insulin signalling with aging.

a, Plasma IGF1 at 20 months. b, Glucose tolerance test at 30 months of age. c, Plasma Fructosamine at 20 months. d, Blood glucose response to 3 hours of ad libitum access to food (chow diet) following an overnight fast at 20 months. Results are mean ± SE with biological replicates (individual mice, n) shown as individual data points or stated within figure. Significance was determined by two-way ANOVA (a-d) or RM two-way ANOVA (b, d) with Sidak post-hoc analysis for within sex effects when a significant sex x treatment interaction was seen. P-values within figures for PI3Ki vs vehicle of the same sex.