Extended Data Fig. 6: Effect of PI3K inhibitor (PI3Ki) on white adipose tissue (WAT) during aging.
a, Plasma leptin at 20 months. b-c, Subcutaneous (s)WAT and visceral (v)WAT area under adipocyte diameter curve (AUC; Fig. 3c). d, Male sWAT genes that expression was significantly altered by PI3Ki at 20 months of age (mRNA by micro-array, n = 4 per group pooled from 2–3 independent mice) and, e, associated top 10 gene ontology biological processes. f, Female sWAT genes that expression was significantly altered by PI3Ki at 20 months of age (mRNA by micro-array, n = 3 per group pooled from 2–3 independent mice) and, g, associated top 10 gene ontology biological processes. i, Significantly altered genes that overlap in male and female sWAT. Results are mean ± SE with biological replicates (sample size, n) shown as individual data points or stated within figure. Significance was determined by two-way ANOVA with sidak post-hoc analysis for within sex effects when a significant sex x treatment interaction was seen (a-c). P-values within figures for PI3Ki vs vehicle of the same sex.
