Fig. 1: Consort diagram for the CALERIE Trial.

DNAm was assayed from blood samples collected at baseline, 12 months and 24 months. Of the n = 197 participants for whom DNAm data were available from baseline and at least one follow-up assessment, baseline to 12-month change was measured for n = 125 CR and n = 66 AL participants and baseline to 24-month change was measured for n = 117 CR and n = 68 AL participants.