Extended Data Fig. 4: Macrophage-derived MANF in muscle regeneration.
From: Aging disrupts MANF-mediated immune modulation during skeletal muscle regeneration
a–c, Quantification, by flow cytometry, of myeloid cells (CD11bpos, a), pro-repair macrophages (F4/80posLy6CLow, b), and ratio of pro-repair to pro-inflammatory macrophages (LyC6Low/LyC6High, c) in regenerating quadriceps (QC) muscles of tamoxifen treated Manffl/fl and ManfCx3cr1Δ mice at 3dpi (n = 4/condition). d, Representative images of cryosections from Tibialis anterior (TA) muscles of Manffl/fl, ManfCx3cr1WT and ManfCx3cr1Δ mice, at 4 and 14dpi, stained with H&E and immunostained with mouse IgG. Asterisks indicate necrotic myofibres. Scale bars: 50 μm for H&E 4dpi; 20μm for IgG 4dpi; 100μm for H&E 14dpi. e, f, Quantification of the average cross-sectional area of central nucleated new myofibres (f) and frequency distribution of new myofibres by size (e), in regenerating TA muscles from Manffl/fl and ManfCx3cr1Δ mice at 14dpi (n = 4 for Manffl/fl; n = 3 for ManfCx3cr1Δ). g, Quantification of the average cross-sectional area of myofibers in non-injured TA muscles from ManfCx3cr1WT and ManfCx3cr1Δ mice (n = 5 for ManfCx3cr1WT; n = 3 for ManfCx3cr1Δ). Data are represented as average ± s.e.m. and each n represents one animal. p values are from two-tailed Student’s t-test. dpi, days post-injury; H&E, Hematoxylin and Eosin; msIgG, mouse Immunoglobulin; CSA, cross-sectional area.
