Extended Data Fig. 8: Transmission electron microscopy analysis MANF-deficient and macrophages.

Representative images of pro-repair macrophages (F4/80^posLy6C^Low) FACS-isolated at 3dpi from QC muscles of Manf^Cx3cr1WT and Manf^Cx3cr1Δ mice, analyzed by TEM. Scale bars: 2 μm Quantifications of these images, for independent cells, are shown in Fig. 6c–e. The quantification includes 54 macrophages isolated from Manf^Cx3cr1WT mice and 42 macrophages isolated from Manf^Cx3cr1Δ mice. Macrophages were sorted from n = 3 animals/condition in 2 independent experiments (experiment 1: n = 1/condition; experiment 2: n = 2/condition, pooled). Samples obtained in each experiment were processed independently for analysis.