Extended Data Fig. 2: Effects of aging and MANF-deficiency in the cellular response during muscle regeneration.

a, d, Gating strategy used in flow cytometry analysis of (a) CD45^p°^s immune cell population, endothelial cells, FAPs and MuSCs; and (d) myeloid cells (CD11b^p°^s), pro-repair macrophages (F4/80^posLy6C^Low), pro-inflammatory macrophages (LyC6^High), and neutrophils (F4/80^negLy6G^pos). b, c, e, f, Quantification, by flow cytometry, of FAPS (b, n = 3/condition), endothelial cells (c, n = 3/condition) and neutrophils (e, n = 7 for Manf^R26WT; n = 6 for Manf^R26Δ) in regenerating muscles of Manf^R26WT and Manf^R26Δ mice at 3dpi; and neutrophils (f, n = 4/condition) in regenerating muscles of yg (2–6 mo) and old (22–24 mo) wt (C57BL/6) mice at 3dpi. g–i, Quantification, by flow cytometry, of myeloid cells (CD11b^pos, g), pro-repair macrophages (F4/80^posLy6C^Low, h) and pro-inflammatory macrophages (Ly6C^High, i) in regenerating muscles of yg (2–6 mo) and old (22–25 mo) wt (C57BL/6) mice at 2 and 3dpi (n = 4 for yg and old 3dpi; n = 5 for old 2dpi; n = 6 for yg 2dpi). Data are represented as average ± s.e.m. and each n represents one animal. p values are from two-tailed Student’s t-test. FAPs, Fibroadipogenic progenitors; MuSCs; Muscle Stem Cells; yg, young.

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