Extended Data Fig. 4: Cold-induced PA28γ/PSME-3 attenuates aggregation of intestinal IFB-2 during aging.
a, Western blot analysis of IFB-2 protein levels in wild-type adult animals cultured at the indicated temperatures after development. Graph represents the relative percentage values of IFB-2 protein levels (corrected for α-tubulin loading control) to 20 °C day 1-adult worms (mean ± s.e.m., n = 3 independent experiments). b, Filter trap analysis with antibody against IFB-2 of wild-type worms at different ages. Representative of four independent experiments. c, Western blot analysis of IFB-2 protein levels in day-10 adult wild-type worms upon psme-3 RNAi at cold temperature (15 °C). Graph represents the relative percentage values of IFB-2 protein levels (corrected for α-tubulin loading control) to Vector RNAi (mean ± s.e.m., n = 5 independent experiments). d, Filter trap with anti-IFB-2 antibody of day-10 adult wild-type worms upon psme-3 RNAi at the indicated temperatures. Representative of three independent experiments. In all the experiments, worms were raised at 20 °C and then shifted to the indicated temperatures after development. RNAi was initiated at day 1 of adulthood. All the statistical comparisons were made by two-tailed Student’s t-test for paired samples. P value: *P < 0.05, NS = not significant (P > 0.05). All the significant changes were also significant after correction for multiple testing by FDR approach (q value < 0.05). See Source Data for exact P and q values.
