Extended Data Fig. 8: iPSC-derived motor neurons have higher levels of basal trypsin-like activity compared with HEK293 cells at standard temperature.
a, Trypsin-like proteasome activity comparing human HEK293 cells and iPSC-derived motor neurons at standard temperature (37 °C). Bar graph represents the mean ± s.e.m. (relative slope to HEK293, n = 3 independent experiments). b, Caspase-like proteasome activity at standard temperature (37 °C). Graph represents the mean ± s.e.m. (relative slope to HEK293, n = 3 independent experiments). c, Chymotrypsin-like proteasome activity at standard temperature (37 °C). Graph represents the mean ± s.e.m. (relative slope to HEK293, n = 3 independent experiments). d, Cold temperature (36 °C) does not induce changes in the caspase-like proteasome activity of ALS iPSC-derived motor neurons (mean ± s.e.m. of the relative slope to 37 °C, n = 3 independent experiments). e, Cold temperature does not induce changes in the chymotrypsin-like proteasome activity of ALS iPSC-derived motor neurons (mean ± s.e.m. of the relative slope to 37 °C, n = 3 independent experiments). All the statistical comparisons were made by two-tailed Student’s t-test for paired samples. P value: **P < 0.01, ****P < 0.0001, NS = not significant (P > 0.05). See Source Data for exact P and q values.
