Extended Data Fig. 5: Confocal image analysis of the effect of CISH on mitochondria and MVBs. | Nature Aging

Extended Data Fig. 5: Confocal image analysis of the effect of CISH on mitochondria and MVBs.

From: CISH impairs lysosomal function in activated T cells resulting in mitochondrial DNA release and inflammaging

Extended Data Fig. 5

a, b, Naïve CD4+ T cells from a young and an older individual were transfected with indicated siRNA and activated for 3 days. Cells were co-stained with anti-CD63 (as MVB marker) and with anti-LC3B (as autophagosome marker) (a) or anti-COX IV (as mitochondria marker) (b). Confocal images representative of two independent experiments are shown. Scale bars, 1 μm.

Back to article page