Extended Data Fig. 8: Effect of CISH on T cell responses in vivo. | Nature Aging

Extended Data Fig. 8: Effect of CISH on T cell responses in vivo.

From: CISH impairs lysosomal function in activated T cells resulting in mitochondrial DNA release and inflammaging

Extended Data Fig. 8

a–d, SMARTA cells were transduced and adoptively transferred followed by LCMV infection as described in Fig. 8. Viral RNA levels in the spleen were determined on day 6, n = 10 (a). Frequencies of SMARTA cells with indicated phenotypes in CD4+ donor cells (b–d) on day 6 after LCMV infection (n = 6). e, Mice were reconstituted with shCish or shCtrl retrovirally transduced naïve OT-II CD4+ T cells (n = 3). Frequencies and absolute numbers of TFH and non-TFH OT-II cells in the spleen on day 8 after OVA immunization. Data are presented as mean ± s.e.m. Comparison by two-tailed unpaired t-test (a–e). *P < 0.05; NS, not significant.

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