Extended Data Fig. 9: Confirmatory studies with a distinct shRNA to silence Cish. | Nature Aging

Extended Data Fig. 9: Confirmatory studies with a distinct shRNA to silence Cish.

From: CISH impairs lysosomal function in activated T cells resulting in mitochondrial DNA release and inflammaging

Extended Data Fig. 9

a-d, 1 ×105 Cish shRNA#2 (5′-AAAACAAGTGTTAGAACACAA-3′) or control shRNA retrovirally transduced Amcyan+ naïve SMARTA CD4+ T cells were adoptively transferred into CD45.2+ naïve recipients followed by LCMV infection. a, CISH and ATP6V1A protein expression in transduced cells before adoptive transfer. b, Serum mtDNA copies at day 6 after LCMV infection. c, Serum proinflammatory cytokines levels on day 6 after LCMV infection. d, Gene expression of key inflammatory markers in splenocytes on day 6 after infection. Data are from one experiment with 3–4 mice per group. Data are presented as mean ± s.e.m. Statistical significance by one-way ANOVA followed by Tukey’s multiple comparison test (b and d) or two-tailed unpaired t-test (c). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; NS, not significant.

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