Extended Data Fig. 6: Analysis of metabolism-associated genes in young GCs.
From: Granulosa cell mevalonate pathway abnormalities contribute to oocyte meiotic defects and aneuploidy
a, Violin plots showing the average number of detected genes (FPKM > 1) in young and aged GCs at different stages. YGC, YFGC, YMIGC, and YMIIGC: GCs from young mice. OGC, OFGC, OMIGC, and OMIIGC: GCs from aged mice. The data are shown with at least three independent experiments each group. b, PCA plot of young and aged GCs of different stages based on gene expression patterns separated by PC1 and PC2. The size and color of the dots represent the stage and age of the GCs, respectively. c, Proportions of lipid-associated genes among the highly expressed metabolic genes at each stage. d, KEGG map of MIGC stage-specific highly expressed genes. Many genes highlighted with red rectangles are involved in steroid biosynthesis. 1.14.1417: SQLE; 5.4.99.7: LSS; 1.3.1.72: DHCR24; 1.14.14154: CYP51; 1.3.1.70: TM7SF2; 1.14.189: MSMO1; 1.1.1.170: NSDHL; 5.3.3.5: EBP; 1.14.19.20: SC5D; 1.3.1.21: DHCR7. e, Heatmap showing the expression levels of MVA pathway-associated genes in the four GC subtypes. The value for each gene is row-scaled Z score. GC, granulosa cell.
