Extended Data Fig. 9: EGF signaling is regulated by the MVA pathway in GCs.
From: Granulosa cell mevalonate pathway abnormalities contribute to oocyte meiotic defects and aneuploidy
a, Expression levels of meiosis signal-associated genes in the four GC subtypes in 6-week-old mice. The value for each gene is row-scaled Z score. b, Number of potential ligand–receptor pairs in GCs and oocytes predicted by CellPhoneDB 2 in young and aged mice. c, Number of ligand–receptor pairs in GCs and oocytes at each stage in young and aged mice. d, mRNA levels of LHR, AREG, and EREG in GCs from young (n ≥ 17) and aged (n ≥ 16) donors. e, Western blot analysis of MVK, LHR and AREG expression in KGN cells treated with atorvastatin or GGOH. The data are shown with three independent experiments. For bar graphs in d,e, data are presented as mean ± SEM. Statistical significance was determined by two-sided unpaired t-test; exact P values are labeled above the graphs.
