Fig. 4: Blockade of IL-17A/F function in aging mice leads to a delay in age-associated skin traits.
From: Targeting lymphoid-derived IL-17 signaling to delay skin aging
a, Diagram showing anti-IL-17A/F treatment workflow. Created with BioRender.com. b, UMAP representation of all CD45+ and CD45–EpCAM– cells analyzed by 10X scRNA-seq obtained following IL-17A/F blockade. c, Plot of selected GO categories belonging to BP analysis for genes upregulated (left) and downregulated (right) after IL-17A/F-blocking treatment in epidermal cells. d, Dot plot showing quantification of cornified layer thickness in aged/IgG control, aged/anti-IL-17A/F-treated and adult mice. Each dot represents the average of ten measurements per mouse (n = 8 mice, collected in two independent experimental replicates pooled) performed on H&E stainings of skin sections. Horizontal lines represent the median of individual values per age and condition. P values were calculated by two-tailed Mann–Whitney U-test. e, Representative images of H&E staining of adult, aged/IgG control and aged/anti-IL-17A/F-treated mice. Scale bars, 50 μm. Cornified layer thickness (black arrowheads) was measured using scaled images.
