Extended Data Fig. 1: Sorting strategy and quality controls of scRNA-seq of adult and aged dermis.
From: Targeting lymphoid-derived IL-17 signaling to delay skin aging
a, Flow cytometry plots representative of the sorting strategy followed to obtain CD45+ and CD45–/EpCAM– cells for 10× scRNA-seq. b, UMAP representation of CD45+ Lymphoid (upper panel), CD45+ Myeloid (middle panel) and CD45- (lower panel) cells showing replicate contribution, batch effect correction and good sample mixing calculated with LISI(Korsunsky, Millard et al. 2019). c, Bar plots showing percentage of total cells divided into each cell type per replicate, CD45+ Lymphoid (left panel), CD45+ Myeloid (middle panel) and CD45- (right panel) cells.
