Extended Data Fig. 5: Gene expression of cell adhesion and migration pathways in NSC lineage during aging by single cell RNA-seq.

a-e, Violin plots of the distribution of single cell cumulative expression profiles of genes within the ‘Cell-matrix adhesion’ GO category (GO:0007160) (a), ‘Adherens junction organization’ GO category (GO:0034332) (b), ‘Response to forskolin’ GO Category (GO:1904322) (c), ‘Cell-cell adhesion mediated by cadherin’ GO Category (GO:0044331) (d), and ‘Cell-cell adhesion (plasma membrane)’ GO Category (GO:0098742) I for young and old qNSCs/Astrocytes (left) and aNSCs/NPCs (right) from single cell RNA-seq data (see Methods). Each overlaid dot represents the cumulative expression of genes within the GO category in a single cell. f-j, Violin plots of the distribution of single cell cumulative expression profiles of genes within the ‘Cell adhesion’ GO Category (GO:0007155) restricted to cadherins (f), ‘Homophilic cell-cell adhesion (plasma membrane)’ GO Category (GO:0007156) (g), ‘Olfactory bulb interneuron differentiation’ GO Category (GO:0021889) (h), ‘cAMP mediated signaling’ GO Category (GO:0019933) (i), and ‘Positive regulation neuron migration’ GO Category (GO:2001224) (j) for young and old qNSC/Astrocyte (left), aNSC/NPC (middle), and neuroblasts (right) from single cell RNA-seq data (see Methods). Each overlaid dot represents the cumulative expression of genes within the GO category in a single cell. k, Gene expression trajectories as a function of age of select cell adhesion genes (Alcam, Ctnnd2, Itgb8) in qNSCs/Astrocytes from single cell RNA-seq dataset (see Methods). Shaded region represents 95% confidence interval. l, Gene expression trajectories as a function of age of select cell adhesion genes (Alcam, Lsamp, Ntm) in aNSCs/NPCs from single cell RNA-seq dataset (see Methods). Shaded region represents 95% confidence interval. All statistical comparisons made using a two-tailed Mann-Whitney test.