Extended Data Fig. 8: The epigenetic program of DN ILC3s and NKp46+ ILC3s.
(a, b) Heatmap for genome-wide distribution of Cxxc1-binding signals at peak centers in DN ILC3s (a) and NKp46+ ILC3s (b) sorted from control and Cxxc1-deficient mice by CUT&Tag. (c) Donut chart showing the percentages of Cxxc1-binding at promoter regions, gene body regions, or intergenic regions. (d, e) Heatmaps illustrating enrichment of H3K4me3 CUT&Tag signals at all gene promoters ( ± 3 kb of TSS) in DN ILC3s (d) and NKp46+ ILC3s (e). (f) Donut chart showing the percentages of H3K4me3 peaks at promoter regions, gene body regions, or intergenic regions. Peak annotation was performed by HOMER. UTR, untranslated region. (g, h) Venn diagram showing genes with reduced enrichment of Cxxc1 and H3K4me3 modification in the indicated cells in the graphs (Chi-squared test was used to calculate the P values. Adjusted absolute log2fc value > 0.25 and adjusted P value < 0.05). (i) Overlaid histograms show expression of indicated protein in aged mice (red), control mice (blue) and Isotype (grey). (j) Overlaid histograms show expression of indicated protein in Cxxc1f/f RorcCre mice (red), control mice (blue) and Isotype (grey). The data are representative of two independent experiments (i) and four independent experiments (j).
