Extended Data Fig. 2: Acute klotho treatment does not alter the FGF23-klotho endocrine system, renal phosphate, or vitamin D (linked to Fig. 1f,g).
(a) Paradigm for serum and urine profiling. Young mice (male, age 4 months; n = 4-5 mice per group) were treated with either Veh or Klotho (s.c. 10 μg/kg) followed by serum and urine collection and profiling. (b) Urine ratio of phosphorus to creatinine in urine between groups (n = 4 mice for Veh group; n = 5 mice for Klotho group). (c) Serum Phosphorus levels between groups (n = 5 mice per group). (d) Serum 25-Hydroxyvitamin D levels between groups (n = 5 mice per group). (e) Serum FGF23 levels between groups (n = 5 mice per group). Data are presented as means ± SEM.
