Extended Data Fig. 8: UA in vitro treatment modulates T cell function and mitochondrial profiles.
a, Splenocytes derived from old mice were activated at day0 and restimulated at day5 with CD3/CD28 beads cultured with 5 μM UA. b, Analyses of mitochondrial activity (TMRM) and mass (MTG, mitotracker Green) in CD8+ and CD4 + T cells measured as mean fluorescence intensity (MFI). c, Analyses of the proportion of cells INFγ+TNFα+ upon restimulation with CD3/CD28 beads at day 5 (n = 4; values are mean ± s.e.m.; two-tailed paired Student’s t test; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001).
