Extended Data Fig. 9: UA modulates mitochondrial recycling in HSCs.
a, Analysis of mitophagy induction in HSCs purified from mito-QC mice measured as MFI ratio between GFP and mCherry signals(P-value = 0.0037). (n = 4; two tailed paired Student’s t test; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001). b, Expression of CD150 measured by mean fluorescence intensity (MFI) in young and old HSCs (P-value = 0.0009)(n = 4; values are mean ± s.e.m.; two-tailed Student’s t test; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001). c, HSCs derived from Mx1-PARK2 mice (CD45.2) were transplanted in lethally irradiated recipient mice (CD45.1/2) together with total bone marrow competitor cells (CD45.1).d, Blood donor chimerism of primary transplant at the indicated timepoint. e, Lymphoid vs myeloid compartments of primary transplants at the indicated timepoints (Ctrl vs PARK2-/- P-value = 0.0002; Ctrl vs PARK2-/- UA P-value = 0.0006) (n = 10; values are mean ± s.e.m.; two-way ANOVA; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001).
