Extended Data Fig. 2: Donor chimerism analysis in the bone marrow.
a, Lymphoid vs myeloid compartments of primary transplants in peripheral blood at the indicated timepoints. b, Analysis of donor derived contribution for lymphoid and myeloid lineages in bone marrow at the endpoint of primary transplant (Lymphoid: young ctrl vs old ctrl P-value = 0.0003; old ctrl vs old UA P-value = 0.0011; myeloid: young ctrl vs old ctrl P-value = 0.0002; old ctrl vs old UA P-value = 0.0006;). c, Analysis of donor derived HSCs at the endpoint of primary transplant. d, Analysis of donor derived HSCs at the endpoint of secondary transplant (n = 10; values are mean ± s.e.m.; two-tailed Student’s t test; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001). e, HSCs isolated from bone marrow of young mice were cultured for three days in the presence of 20μM UA and transplanted in lethally irradiated primary recipient together with bone marrow derived from competitor mice. Blood donor chimerism analyses of primary transplant at indicated timepoints (n = 10; values are mean ± s.e.m.).
