Extended Data Fig. 5: Hematopoietic stem and progenitor analyses of old mice supplemented with UA.

a, Gating strategy to identify hematopoietic stem and progenitor populations from the BM for endpoint analysis. HSCs: Lineage⁻cKit⁺Sca1⁺ (LKS) CD150⁺CD48⁻; Multipotent progenitors (MPPs): LKS CD150⁻, Common lymphoid progenitor (CLP): CD127⁺ cKit^low Sca1^low; Committed progenitors (cKit⁺) include common myeloid progenitors (CMPs: Lineage⁻cKit⁺Sca1^–(KLS^–) FcRlowCD34⁺), granulocyte–macrophage progenitors (GMPs: KLS-FcRlowCD34 + ), and megakaryocyte-erythroid progenitors (MEPs: KLS^–FcR^–CD34^–). b, Analyses of lymphoid vs myeloid compartments of primary transplants at the indicated timepoints (n = 10; values are mean ± s.e.m). c, Donor chimerism analysis of secondary recipient mice in peripheral blood (Total: 4 weeks P-value = 0.0013; 8 weeks P-value < 0.0001; 12 weeks P-value = 0.0003; Lymphoid: 4 weeks P-value < 0.0001; 8 weeks P-value < 0.0001; 12 weeks P-value = 0.0002; Myeloid: 4 weeks P-value = 0.0089; 8 weeks P-value = 0.0049; 12 weeks P-value = 0.0153) (n = 5; values are mean ± s.e.m.; two-tailed Student’s t test; * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001).

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