Extended Data Fig. 5: Physiological benefits of senolytic ABT-737 and VPF treatments in naturally aged mice.
From: The YAP–TEAD complex promotes senescent cell survival by lowering endoplasmic reticulum stress
a, Pictures of the 24 m.o. mice from the different groups described in Fig. 4a at the end of the experiment. b, UMAP clustering of the scRNA-seq performed in lungs from the indicated groups. c, Feature plot displaying Cdkn2a mRNA expression in the indicated experimental groups. Each plot was obtained by merging the two samples sequenced for each experimental group, shown in (b). d, Plots displaying the signals obtained through flow cytometry analysis of single-cell lung suspensions from the indicated groups. FSC-H axis represents the signals obtained for the forward scatter, while the PE-Cy7-A axis corresponds to CD45 staining with such fluorophore. Cells considered CD45+ are colored in blue, and the percentages are specified on the top right corner of each box. e, Dot plot displaying the association of the indicated cell types and conditions with the top 15 transcripts from Hallmark: Inflammatory Response gene set. The size of the dots represents the percentage of cells expressing such transcript while the intensity of red indicates the relative expression value. f, GSEA plots displaying the association of the indicated cell types from Old DMSO condition (compared to the rest of the experimental groups) with the indicated gene sets (Reactome: Translation; Safford T Lymphocyte Anergy; Reactome: Interleukin 6 Family Signaling; Reactome: Antigen Activates B cell Receptor BCR Leading To Generation Of Second Messengers). g, h, Masson’s trichrome (MTC) staining performed in liver and kidney from the indicated groups, representative images of MTC staining (g) in blue, and quantification of the blue area present at each sample divided by the total area in red (h). Scale bar, 200 µm. i, j, Serum analysis of blood urea nitrogen (i) and AST (j) levels for the indicated experimental groups. Plots in (h–j) represent the means and the individual values as dots ± s.d. of the included mice (more details in Supplementary Table 6); significance (*P < 0.05, **P < 0.01, ***P < 0.001) was calculated using one-way ANOVA.
