Extended Data Fig. 1: Analysis of immune cells in Lamin A/C CKO mice.
From: Nuclear envelope disruption triggers hallmarks of aging in lung alveolar macrophages
(A) Flow cytometric analysis of WT vs Lamin A/C CKO macrophage and dendritic cell (DC) populations in spleen, with gating strategies for each population represented by colored gating. Left, representative example of the gating strategy. Right, percentage of each population of live spleen cells and absolute counts (n = 4 mice, combined from 2 independent experiments, bar indicates mean ± SD, one-way ANOVA with Šidák test). (B) Flow cytometric analysis of WT vs Lamin A/C CKO Natural killer, T and B cell populations in spleen and lymph nodes, with gating strategies for each population represented by colored gating. Top, representative example of the gating strategy. Bottom, percentage of each population of live spleen or lymph node cells and absolute counts (spleen, n = 6 WT and 5 Lamin A/C CKO mice for NK and T cells analysis, 4 WT and 4 Lamin A/C CKO mice for DCs, macrophages and B cells; lymph nodes: n = 8 WT and 6 Lamin A/C CKO mice for NK and T cells analysis, 6 WT and 5 Lamin A/C CKO mice for B cells combined from 4 independent experiments, bar indicates mean ± SD, one-way ANOVA with Šidák test). (C) Flow cytometric analysis of WT vs Lamin A/C CKO to analyze hematopoietic stem cell (HSC) and plasmacytoid dendritic cell populations in bone marrow, with gating strategies for each population represented by colored gating. Top, representative example of the gating strategy. Bottom, percentage of each population of live bone marrow cells and the absolute counts (n = 4 WT and 4 Lamin A/C CKO mice for pDC analysis, n = 6 WT and 6 Lamin A/C CKO mice for all the other populations, combined from 3 independent experiments, bar indicates mean ± SD, one-way ANOVA with Šidák test). Acronyms used: Short term HSC (ST-HSC), Multipotent hematopoietic progenitor (MPP), common myeloid progenitor (CMP), common lymphoid progenitor (CLP), granulocyte macrophage progenitor (GMP), megakaryocyte-erythroid progenitor (MEP), plasmacytoid dendritic cell (pDC).
