Extended Data Fig. 4: Chemotactic microglia migrate toward nonlipidated ApoE after interleukin-33 treatment.
From: The VCAM1–ApoE pathway directs microglial chemotaxis and alleviates Alzheimer’s disease pathology
a,b, Quality control for bead injection. Representative images (a) and quantification (b) showing the bead injection area (Con: n = 12; interleukin-33 [IL-33]: n = 18, two-tailed unpaired Student’s t-test). Scale bar = 50 μm. c,d, IL-33-induced chemotactic microglia migrate towards human ApoE isoforms. Representative images (c) and bar plot (d) showing microglial migration towards BSA-, murine ApoE (mApoE)-, human ApoE3-, and human ApoE4-coated beads after IL-33 treatment (n = 3 per condition; one-way ANOVA with Šidák’s multiple comparisons test). Dotted line indicates bead area. Scale bar = 25 μm. All data are mean ± s.e.m.
