Extended Data Fig. 6: Activated state of microglia cells line BV2, astrocytes cells line MA-c in vitro after IAA treatment (n = 6).
From: Chiral nanoparticle-remodeled gut microbiota alleviates neurodegeneration via the gut–brain axis
Detection of activated (A) M1(CD68+CD86+), (B) M2 (CD206+CD163+) microglia cell line BV2 and (C) A1, (D) A2 astrocyte cell line MA-c by flow cytometry. (E) Detection of activated M1, M2 microglia cell line BV2 and A1, A2 astrocyte cell line MA-c by confocal. Scale bars, 20 μm. CH-223191, the AHR inhibitor. Box plots extend from the 25th to the 75th percentile with the median value shown as a black line in the middle, and whiskers denote the minima and maxima values. Each ‘n’ represents an independent biological sample. One dot represents one sample. Data are represented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. One-way (A-D) ANOVA followed by Tukey’s multiple comparisons test.
