Fig. 1: The co-transcriptional function of CRTC-1 is required for H3K4me3-mediated longevity.
From: The CRTC-1 transcriptional domain is required for COMPASS complex-mediated longevity in C. elegans
a, Volcano plot showing proteins identified during IP analysis of endogenous CRTC-1::3xFLAG tagged strain relative to wild-type non-tagged strain (N2). The dotted line indicates a one-fold change. Four biological replicates. P values by two-sample t-test two-tailed. b, STRING protein–protein interaction network of CRTC-1-binding proteins identified during IP analysis. The network nodes are proteins. The edges represent the predicted functional associations. Red line (presence of fusion evidence), green line (neighborhood evidence), blue line (co-occurrence evidence), purple line (experimental evidence), yellow line (text mining evidence), light-blue line (database evidence) and black line (co-expression evidence). c, Top: schematic of ER–Golgi traffic regulated by the SEC31-binding site of CRTC. Bottom: amino acid sequence localization of the SEC31-binding site and CRISPR edit. d, Top: schematic of co-transcriptional regulation by the CREB-binding domain of CRTC. Bottom: amino acid sequence localization of the CREB-binding domain site and CRISPR edits. e, crtc-1Sec31−/− specifically regulates SREBP1/SBP-1 activation. Intestinal images of GFP::SBP-1 reporter in wild-type, crtc-1Sec31−/− and crtc-1CBD−/− animals. Quantification of GFP::SBP-1 nuclear accumulation; mean ± s.e.m. of n nuclei: wild type (125), crtc-1Sec31−/− (133) and crtc-1CBD−/− (123), pooled from at least three independent experiments, P values by Mann–Whitney test two-tailed. f, crtc-1CBD−/− specifically regulates CRE promoter transcriptional activation. Images of CREp::GFP reporter in neurons of the head in wild-type, crtc-1Sec31−/− and crtc-1CBD−/− animals. Quantification of CREp::GFP expression; mean ± s.e.m. of n heads: wild type (36), crtc-1Sec31−/− (29) and crtc-1CBD−/− (29), pooled from at least three independent experiments, P values by Mann–Whitney test two-tailed. g, Survival curves showing that set-2(ok952) mutants live longer than wild type, and crtc-1Sec31−/− does not suppress this longevity phenotype. h, Survival curves demonstrating that set-2(ok952) mutants live longer than wild type, and crtc-1CBD−/− completely suppresses this longevity phenotype. Survival curves compared by the log-rank (Mantel–Cox) method.
