Extended Data Fig. 1: Aging is associated with a fasting-like transcriptional program (FLTP).
From: Refeeding-associated AMPKγ1 complex activity is a hallmark of health and longevity
a, b) KEGG pathway enrichment analysis of up- (a) and down-regulated genes (b) in young fasted/fed fish with FDR values displayed on a negative log10 scale along the x-axis. c) qPCR analysis of top fasting-regulated genes in young and old fish, young fed (n = 4), young fasted (n = 5), Old fed (n = 8), Old fasted (n = 5). Data values indicate fold change over the average value of young samples. d) Food intake analysis performed at different age points. 7 weeks old n = 9, 8 weeks old n = 8, 10 weeks old n = 9, 12 weeks old n = 9, 16 weeks old n = 7, 18 weeks old n = 7, 21 weeks old n = 5. e) Blood glucose measurements of young and old fish under feeding or fasting regime, Y-fed n = 4, Y-fasted n = 4, O-fed n = 5, O-fasted n = 5. f) Fulton’s index analysis in young and old killifish either fed or fasted. Y-fed n = 10, Y-fasted n = 8, O-fed n = 7, O-fasted n = 8. Data in c-f are presented as mean ± S.D. Significance was obtained by two-way ANOVA followed by Sidak multiple comparison test in c, e, and f, by one-way ANOVA followed by Tukey’s post hoc test in d.
