Extended Data Fig. 5: hsf-1 knockdown alters genes involved in lipid synthesis and breakdown (related to Fig. 5).

(a) Differentially expressed genes in day 1 adult Q40::YFP worms maintained on hsf-1 RNAi vs L4440 control RNAi were analyzed for metabolic pathway enrichment (see methods). Significant categories are color-coded by p-value. (b) Q40::YFP worms maintained on L4440 or hsf-1 RNAi, and accumulation of acdh-1 was assessed by qPCR. Levels of the housekeeping gene pmp-3 were used as a control for normalization. (c) Q40::YFP animals were maintained on L4440 or hsf-1 RNAi in the presence or absence of oleic acid (0.8 mM) until day 1 of adulthood. Worm motility was determined by assessing animal thrashing rate following placement in a drop of M9. (d) OCR was assessed in day 5 adult Q40::YFP worms supplemented with oleic acid (0.8 mM) or maintained on ash-2 RNAi. (e) Changes in genes involved in fatty acid synthesis and breakdown in Q40::YFP worms deficient in hsf-1. Genes with statistically significant differences vs L4440 control are listed in bold. Results are the average (a, e), representative (c) or average ± SEM (b, d) of two (c), three (d), or four (a, b, e) independent experiments. Statistical significance was assessed by one-way ANOVA (B-D); *, p < 0.05; ****, p < 0.0001. Additional experimental information on RNAseq analysis can be found in Supplementary Table 3.