Extended Data Fig. 7: Histone modifications are associated with chromatin accessibility at the promoters of age-dependent genes in aging NSPCs.
a, Heatmap shows fold change (Aged vs. Young) of RNA levels of age-dependent genes and the average signals of ATAC-seq at their regulatory elements. b, Venn diagrams show the overlap of age-dependent genes that have decreased levels of chromatin accessibility and H3K4me3 at the promoters during NSPC aging. Two-sided Hypergeometric test for significant overlap analysis, * p-value = 5.602e-26. c, Venn diagrams show the overlap of age- dependent genes that have decreased levels of chromatin accessibility and H3K27ac signals at the enhancers during NSPC aging. Two-sided Hypergeometric test, * p-value = 3.618e-16. d, Browser tracks of ATAC-seq and ChIP-seq show a similar level of signals at the promoters of NSPC lineage markers and housekeeping genes between the young and aged groups. e, Browser tracks of 3 age-dependent down-regulated genes (Igf2bp3, Twist1 and Otx2) and 2 up-regulated genes (Gpr37l1 and Ifi27) that have changes of ATAC-seq signals and histone modifications at the promoters during NSPC aging.
