Extended Data Fig. 10: Interaction between MLL complex and Cbx3 in aging NSPCs.
a, Mass spectrometry peptide counts (number of unique peptides) of MLL complex components identified in KMT2D immunoprecipitation product from young NSPC nuclear extract. b, Mass spectrometry peptide counts (number of unique peptides) of proteins identified in CBX3 immunoprecipitation product from young NSPC nuclear extract. c, Western blot analysis of endogenous MLL complex components in CBX3 and IgG immunoprecipitation products from the young and aged NSPC nuclear extracts. Bar charts show the relative levels of the indicated proteins in aged versus young IP groups analyzed by Image J software. Error bars are mean ± SD. n = 2 independent experiments, Unpaired two-sided Student’s t-tests. KMT2D: P = 0.0222, RBBP5: P = 0.0015, WDR5: P = 0.6499, CBX3: P = 0.4363. * P < 0.05. *** P < 0.005. ns: not significant.
