Extended Data Fig. 8: Representative flow cytometry gating strategy used in this study.
From: Age-related noncanonical TRMT6–TRMT61A signaling impairs hematopoietic stem cells
(a) Representative flow cytometry showing the gating strategy used to analyze and isolate the hematopoietic stem and progenitor cell populations in mouse bone marrow presented on Figs. 1a-b, e, h, 2b-c, e-m, 3a-c, d, f, i, j, m, 4a, f-g, i-o, 5a, d, 6f-i, 7h, i, and Extended Data Figs. 1e-h, 2a-f, 3a, 4a-l, 6c-d. (b) Strategy used to evaluate the the percentage of B, myeloid, CD4+ T, CD8+ T cells presented on Fig. 1j and Extended Data Fig. 1c-d. (c-d) Strategy used to evaluate the the percentage (c) and lineage distribution (d) of test donor-derived cells (myeloid, B and T cells) presented on Figs. 1g, k-l, 2d, 3h, 5b, e-k, l-m, 6d-e and Extended Data Figs. 1i-j, 5a-i.
