Extended Data Fig. 2: Aged HSCs exhibit altered tRF profile compared to young ones.
From: Age-related noncanonical TRMT6–TRMT61A signaling impairs hematopoietic stem cells
(a) This line plots showing the tRF reads length distribution between young and aged KSL cells. (b) This histogram shows the percentage of the indicated tRF types between young and aged KSL cells. (c) Volcano Plot showing the expression of tRF-5, tRF-3, 5’-tRNA half (5’-tiRNA), 3′ -tRNA half (3’-tiRNA), and internal-tRF between young and aged KSL cells. (d) The scatterplot depicts the tRNA expression in young and aged KSL cells. (e) The histogram showing the expression of tRNA-derived fragments between young and aged KSL cells. The sequence of individual tRF was listed in Table S4. n = 3 independent experiments. (f) The histogram displays the expression of 3’-tiRNA-Leu-CAG in KSL cells carrying indicated shRNA. n = 3 independent experiments. Two-tailed unpaired Student’s t-test was used for statistical analysis in e-f. Data are shown as the mean ± SD in e-f.
